Yeast Fermentation and Expression Service starts at $5,500 to $5,625 (price for 3-L scale)
Service steps
Service specification
Timeline
Plasmid construction
Gene synthesis with the codon optimization and sub-clone
Construct linearization
Timeline: 2-3 weeks
Protein expression test
Transformation of the linearized plasmid into suitable host
Antibiotic screening, select 5-10 positive clones
Expression analysis and identification
250ml amplification and purification test
Tag-free test (if required)
Timeline: 3-4 weeks
Large-scale fermentation and purification
Protein affinity purification, ion-exchange, and gel-filtration (to obtain the desired amounts and purity)
Timeline: 1-10 L 3-4 weeks
QC the purified protein
Timeline: 10-20 L 4-5 weeks
All timelines are approximate. In most cases, we deliver the final pure protein within 8-10 weeks. However, optional steps might prolong the overall timeline slightly.
