Instruments and Screening Platform

 HIGH THROUGHPUT FLOW CYTOMETRY PLATFORM-TMC3 HELIX PARK

• Bio-Rad ZE5 Cell analyzer: a 5-laser detector Flow cytometer designed for a combination of throughput and superior speed and sensitivity for high throughput screening from small particles to cells via tubes to 384-well plate.
• Thermo Scientific high-capacity Incubators, 37C and 4C.
• Agilent ELx405 select Deel Well Washer
• Agilent Microplate Centrifuge: robot-accessible automated centrifuge.
• Agilent MultiFlo FX multimode Dispenser: automate multimode reagent dispenser for 6-1536-well microplates, two head dispensers.
• Formulatrix Mantis: a diaphragm-based chip automated liquid dispenser from 6 to 1536 well plates with minimum dispense of 100nl.
• All peripheral instruments are automated with Universal Robotic UR10 robotic arm.

Cytek Aurora Spectral Flow Cytometer 

• Configured with 3 lasers (488, 567, and 640 nm) and 32 fluorescent channels (increased multi-parametric capability).
• Equipped with automated plate loader for 96-well microplate analysis.
• Provides full imaging spectra, which means each fluorochrome is analyzed across the entire light spectrum (spectral flow cytometry).
• Provides high-resolution data (ultrasensitive optics and low-noise electronics).
• SpectraFlo software extracts autofluorescence background for all samples.

BD Biosciences FACSFusion Cell Sorter 

• Uses electrostatic droplet-based, high-speed technology for isolating any combination of fluorescently labeled cell markers.
• Uses 4 lasers (405 nm, 488 nm, 561 nm, and 640 nm) and 15 filters for experiments requiring up to 15 multicolor analyses.
• Variety of nozzle sizes/pressure configurations for sorting all types of cells including fragile ones.
• Capable of sorting up to 50,000 events/second in a temperature-controlled sample and collection chamber.  
• Certified to perform BSL2 level sorting of single cells into 96 well plates, as well as bulk sorting into two or four tubes. 

BD Biosciences LSRII analytical flow cytometer 

• Equipped with 4 lasers (405 nm, 488 nm, 561 nm, and 640 nm) and 13 filters suitable for experiments requiring up to 13 colors plus forward and side scatter data acquisition.
• Users have access to FlowJo data analysis software for customization data analysis.