The list below summarizes our comprehensive service offerings at the core facility
Custom cloning service (Genomic DNA clone; cDNA clone; genetic engineering)
Site-directed mutagenesis (Single mutation and multiple mutations in a single construct; library of random mutants)
Expression optimization tests
Pilot scale protein expression and purification
Large-scale protein production
Co-expression and purification of protein complexes
Protein refolding from inclusion bodies
Tag removal for purified recombinant proteins
SDS-PAGE & Western Blot assays for expressed recombinant proteins
Size-exclusion chromatography (GE Healthcare’s prepacked SEC columns from analytic scale Superdex increase to production grade Superdex) on ÄKTA Pure system
Affinity chromatography (GE Healthcare’s prepacked affinity columns, i.e. HisTrap HP, StrepTrap HP, GSTrap HP, MBPTrap HP) on ÄKTA Pure system
Endotoxin removal for purified recombinant proteins
Multiple-step protein purification on ÄKTA Pure system
Protein activity assay
Protein crystallization screening, optimization, and scale-up; customers also can rent time on Mosquito LCP and Dragonfly screen optimizer with our supervision and assistance.
Spectroscopy-based biophysics assays (CD, ITC, and SPR); customers also can rent time on MicroCal PEAQ-ITC and Biacore T200 with our supervision and assistance.
